![]() and item discovery is lowered - human/hollow does not affect). (Tried to kill enemy, then die, then kill respawned, then die. It looks like item discovery is lowered for enemy after you kill same enemy multiple times (after respawn). Human/hollow form does not affect item discovery. ![]() Soul memory and currently collected souls do not affect item discovery calculation / drop. This will increase the drop rate of the other items when killing this individual falconer. For example if you kill a falconer and it drops the falconer gloves that falconer will never drop the falconer gloves again until you enter a new NG cycle either by beating the game or using a Bonfire Ascetic. Some items will only be dropped by an enemy once. Then you will get Hollow Infantry Boots as drop. If it is first Infantry in the Forest o Fallen Giants that has these drops: Index 3 is 4th item in drop items list (counting from 0). In our example you will get 3 (+1 +1 +1 then stop before 4th +1). And you get the index of your drop that can be from 0 to 9 (10 total). Once sum of tiers becomes higher or equal to random number then addition stops. Now let's say your random number generated as 1000. But your "addition" is always the same as you have from equipped items. If enemy drop rate should be more rare then formula addition steps are lowered and base numbers at the end are lowered too. It seems that the formula is based on the enemy you kill. If your addition is 265 (all from items) then you will get 4220. If your addition = 100 like from Symbol of Avarice: Let's call your additional item discovery from equipped items "addition."įormula is tested on Hollow Infantry. Total item discovery is calculated as follows in 10 maximum steps (they also equal to drop tiers): ![]() If this random number is higher than your item discovery then you don't get any drop at all. Just a random number is used to select a drop item from the list of drop items assigned to enemy. The higher your item discovery, the more chance you will get a drop.Īll items have the same rate of drop (no different specified). ![]() It looks like item discovery only affects whether you will get a drop or not. Nearly all enemies in game (except bosses, and also known - hollows in Things Betwixt) can have up to 10 possible drop items. So best stack is Symbol of Avarice + Covetous Gold Serpent Ring+2 + Prisoner's Tatters + Watchdragon Parma + Rusted Coin + Bonfire Intensity 8 = 740. They do not represent percent of item discovery increase. These are just base points that are used in item discovery calculation. The increase begins at +150 for bonfire intensity 2 and caps at +375 for bonfire intensity 8. (5 per second, can be reduced with the Ring of Restoration and Charred Loyce Shields))īurning Bonfire Ascetic will increase item discovery on enemies in that area. Symbol of Avarice = 100 (also increases soul absorption, but you gradually lose health The actual amount each item increases item discovery is: Update: See full-scale test results here. In Dark Souls II, the amount of item discovery you have is hidden from view, but there are certain items that display that they increase your item discovery. It was a normal stat displayed on your character screen in Dark Souls that was adjusted due to different factors like items you wore and whether you were human or hollow. Item discovery is a hidden stat in Dark Souls 2 that represents the rate at which you discover items.
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![]() Read "fix" as: don't blame parents for any abuse no one is responsible or guilty for abusing you just get over it there is no choice it's just an event from the past rearing its head and it's your fault if you let it get to you you have to make amends for the past. ![]() Mind you that not everyone can fix or wants to "fix" the relationship with their parents, especially if there's severe abuse involved. Honestly, the author focuses too much on how you need to fix and have a relationship with your parents and that if you fix this relationship then you will never be mentally ill again. While the first few chapters provide some useful information about how genetics and epigenetics play a role in our health, the author goes off into kookyville with his personal therapy. Uncovering our core language can free us from reliving traumas that don’t belong to us.” “As creator of the Core Language® Approach, I’ve spent the last 20 years observing how language and trauma intersect-how patients’ presenting symptoms, specifically the language they use, can lead us to the root of the issue, often to a trauma in the family history or in early childhood. Mark has published poetry in The New Yorker. His graduate work at the University of Pittsburgh and at the University of Arizona was also in English. ![]() Mark is a Summa Cum Laude graduate in English and Psychology from the University of Pittsburgh. Mark specializes in working with depression, anxiety, obsessive thoughts, fears, panic disorders, self-injury, chronic pain and persistent symptoms and conditions. His book IT DIDN’T START WITH YOU: How Inherited Family Trauma Shapes Who We Are and How to End the Cycle (Viking/Penguin) is the winner of the 2016 Silver Nautilus Book Award in psychology. He has taught at the University of Pittsburgh, the Western Psychiatric Institute, Kripalu, The New York Open Center, The Omega Institute, The California Institute of Integral Studies. A sought-after lecturer, he leads workshops at hospitals, clinics, conferences, and teaching centers around the world. It Didn’t Start With You is a transformative approach to resolving longstanding difficulties that in many cases, traditional therapy, drugs, or other interventions have not had the capacity to touch.ĭirector of The Family Constellation Institute, The Inherited Trauma Institute and The Hellinger Institute of Northern California, Mark is North America’s leader in Inherited Family Trauma. And visualization, active imagination, and direct dialogue create pathways to reconnection, integration, and reclaiming life and health. Techniques for developing a genogram or extended family tree create a map of experiences going back through the generations. Diagnostic self-inventories provide a way to uncover the fears and anxieties conveyed through everyday words, behaviors, and physical symptoms. ![]() It Didn’t Start with You offers a pragmatic and prescriptive guide to his method, the Core Language Approach. These emotional legacies are often hidden, encoded in everything from gene expression to everyday language, and they play a far greater role in our emotional and physical health than has ever before been understood.Īs a pioneer in the field of inherited family trauma, Mark Wolynn has worked with individuals and groups on a therapeutic level for over twenty years. Even if the person who suffered the original trauma has died, or the story has been forgotten or silenced, memory and feelings can live on. It Didn’t Start with You builds on the work of leading experts in post-traumatic stress, including Mount Sinai School of Medicine neuroscientist Rachel Yehuda and psychiatrist Bessel van der Kolk, author of The Body Keeps the Score. The latest scientific research, now making headlines, supports what many have long intuited-that traumatic experience can be passed down through generations. The evidence is the roots of these difficulties may not reside in our immediate life experience or in chemical imbalances in our brains-but in the lives of our parents, grandparents, and even great-grandparents. A groundbreaking approach to transforming traumatic legacies passed down in families over generations, by an acclaimed expert in the fieldĭepression. ![]()
![]() Dual Filter architecture includes more than 34 Filter algorithms in series or parallel.Deeper FM/Ring Modulation capabilities for aggressive timbres.ORB circular motion interface allows infinitely-shifting variations.Wavetable Synthesis – Each waveform is a Morphing Wavetable.Sound Lock™ feature gives endless useful variations by locking sound aspects while browsing.Creative “Psychoacoustic” sounds from the renowned Spectrasonics sound design team.58 incredible FX Units, which are fully integrated and can be modulated.Powerful Granular Synthesis for amazing transformations.Hardware Library with distinct ‘classic analog’ flavor.Sound Match™ feature instantly locates any related sounds in the library.Over 500 DSP Waveforms for the Synth Oscillator.State of the Art Arpeggiator with many unique features.Audio Import – Use your own audio file as a soundsource.Ground-breaking Hardware Synth Integration allows hands-on control of Omnisphere.Now includes over 14,000 inspiring sounds for all types of music production.Omnisphere’s amazing sound library is constantly growing and being pushed in fresh directions by the award-winning Spectrasonics Sound Development Team. Since the beginning, Omnisphere has been the sonic weapon of choice for creative players and producers in search of next level sounds on hit records, bestselling games and Oscar-winning films. The modernized GUI is now easier to use and resizable to fit comfortably into your workspace. Omnisphere supports a newly redesigned, high resolution interface with support for HiDPI displays. You can use your own audio file as a soundsource in Omnisphere, simply by dragging it onto the interface! Go wild with using the new Granular Synthesis, Harmonia, Innerspace and many other creative tools inside Omnisphere! High Resolution Interface 8 LFOs, 12 ENVs, 34 Filter Types per part.Best of all, the new sounds are available for ALL users, regardless of using the hardware or not! Vastly Expanded Synthesis Engine ![]() These inspiring new sounds feature a wide range of categories and many have a distinctly ‘classic’ flavor! Notably, the 2.6 update takes Eric’s classic sound design work from the original 1987 Roland D-50 into completely new sonic territory with Omnisphere’s vast synthesis capabilities. Each hardware profile has a corresponding set of sounds in the Hardware Library which were specially designed using that hardware synth as an Omnisphere 2.8 controller. Omnisphere 2.8 includes a stunning new “Hardware Library” with over 1,600 new patches created by Eric Persing and the renowned Spectrasonics Sound Development team. Virtual instrument users can now experience the joy of the hardware synth workflow and hardware synth users can fully expand their capabilities into the vast sonic world of Omnisphere 2.8! Greatly Enhanced Arpeggiator Simply put, this ground-breaking feature makes using Omnisphere feel just like using a hardware synth! By bridging the physical experience gap between software and hardware, users gain intuitive control of Omnisphere 2.8 by using the familiar layout of their supported hardware synth. This remarkable innovation transforms over 65 well-known hardware synthesizers into extensive hands-on controllers that unlock Omnisphere’s newly expanded synthesis capabilities. Omnisphere 2.8 is the only software synth in the world to offer a Hardware Synth Integration feature. This award-winning software brings many different types of synthesis together into one amazing-sounding instrument that will spark a lifetime of exploration. Top Artists all over the world rely on Omnisphere as an essential source of sonic inspiration. Omnisphere 2.8 is the flagship synthesizer of Spectrasonics – an instrument of extraordinary power and versatility. Simply put, this ground-breaking feature makes using Omnisphere feel just like using a hardware synth! By bridging the physical experience gap between software and hardware, users gain intuitive control of Omnisphere by using the familiar layout of their supported hardware synth.Omnisphere 2.8.5c + CoreLibrary VST3 AU AAX STANDALONE Hardware Synth Integration Omnisphere is the only software synth in the world to offer a Hardware Synth Integration feature. ![]() Team R2R | | 88.8MB Omnisphere® is the flagship synthesizer of Spectrasonics - an instrument of extraordinary power and versatility. The interesting detail is in how quickly the dogs located the person. When it came to only one cue at a time, the sight, sound, and smell situations were each about equally effective in helping the dog, although none was as effective as all three cues at once. They timed the dog in each situation to see how long it took him to find the person in the testing room and get a treat.Īs you would expect, the dogs found the person almost every time when they had all three cues – sight, sound, and smell. They tested 11 dogs in an experiment that allowed the dogs to detect the presence of a person by smell only, sight only, sound only, or using all three senses at once. Megumi Fukuzawa and Marina Watanabe looked at how sight, sound, and smell can cue dogs to a person’s presence. Have you ever wondered which would be easier for him to understand, spoken words or visual cues? What is the most effective way of communicating with your dog? Recent research from Nihon University, in Japan, helps answer this question. But no doubt, you do a lot of talking to your dog, too. (5) They don't hear the doorbell (or the UPS truck).You may have seen deaf dogs responding to the hand signals of their owners, and perhaps, you use hand signals with your own dog. (4) They don't hear other dogs barking in the neighborhood, so they don't chime in. (3) They watch body language more acutely than hearing dogs. (2) They sleep soundly - most noises will not wake them, but the VIBRATIONS from others might. I do involve owners in the handling of their pets, both in studio and on location, but for reinforcement purposes, I like to understand how they "speak" to their dog.ĭeaf dogs bring certain advantages to the table: I will ask a client to show me the nuances of their own signals, when working with their dog to capture a great photograph. ![]() I have found that clients who have deaf dogs, often have a series of hand signals they use, much like ours, to make requests. Using their signs, and body language, they have no problem complying with STAY STILL requests for portraits. ![]() I rarely have a problem photographing our deaf dogs. ![]() He is showing her the reward she will receive when she completes the task. Stanton is asking Talley to STAY, and has already started to back up. Lacy shows Talley that sign, above, when she has met the command requested. In our house, thumbs up means "good" or "okay" to our deaf dogs. Pets and children also have to be taught how to respect each other. The video below is a good representation of most of the signs we use, and I like that a child is involved in the process, because I firmly believe kids need to be taught early how to help care for a pet. We parlay some of these very same signs into our deaf-dog vocabulary, as well as American Sign Language and things we just made up to suit our own dogs. ![]() Hand signals are often used in dog training, even for hearing dogs. Signs for quiet, sit, stay, down, wait, outside, eat, finished/no more, no, leave it/drop it, walkies.all of these and more are a part of our daily lives.īut signs aren't so far fetched. It has not only helped us to move forward with our two additional deafies (our Border Collie, Piper, and our recent addition, Talley, an English Setter), but has given us a lot of insight into the importance of routine, schedules, and body language. Burton is credited with being our first bilaterally deaf adoption, and he taught us so much of what we needed to know. Two are still with us, but we lost one (Burton, pictured near the end of this blog) a bit over a year ago at age 11. ![]() Our transformed minds are by grace, not our own wisdom, so we have no place to boast. It is true that our new life in Christ can convict them, Paul gets at this in Ephesians 5:3-21, but we have no business parading a self-righteous attitude in our rejection of homosexuality, divorce, abortion, etc. They hate us because we testify to the truth of Christ and they do not believe (1 Tim 1:13). The world is not to hate us because we are offensive in and of ourselves the offense is in the gospel (1 Cor. There is a big difference between these two. The world will hate Christians because we believe in Christ (John 3:19-20 2 Cor. The world's hatred of the church is not to be because the church represents morality. This is not the origin of ‘culture wars' because the people of God are neither to create their own ‘culture' nor are they to defend a conservative agenda for American (or European or English) culture. God tells us here that he intends the wicked to hate the righteous. ![]() The animosity we experience from unbelievers is sin, but it is divinely ordained hatred. What is important to note is that the enmity does not merely arise, it is specifically said to be set or appointed by God himself. This Hebrew word for ‘enmity' is used just five times in the OT (see Ez 25:15, hostility 35:5 Num 35:21-22, hatred unto murder). Moses places ‘enmity' first for the sake of emphasizing it. The normal structure of a Hebrew sentence begins with the verb but here the noun comes first (enmity). These elements of this first prophecy can be put under three categories: two seeds in humanity, judgment on Satan, and the victory of the Savior. There are seven things we will look at in this verse: (1) enmity between the seeds, (2) the source of the enmity, (3) the identity of the serpent, (4) the meaning of ‘seed,' (5) the identity of the woman's seed, (6) the doom of the serpent's seed, and (7) the suffering of the woman's seed. The gospel is spoken in Scripture for the first time from the Creator and Redeemer himself. In the midst of uttering a curse upon the serpent, God also prophetically announces the first promise of the gospel. God is the first prophetic voice of Scripture.īut v.15 is even more than that. Before God addresses Adam and Eve to curse them for their rebellion against himself in the covenant of works (3:16-19), God curses Satan in what is a foretelling of what will be the history of humanity. God is speaking to the serpent (3:14) about the new order for the universe after Adam's fall into sin (3:1-7). "Enmity I will put between you and the woman, PROTOEVANGELIUM OF GENESIS 3:15 By Dante Spencer
![]() Subscription with a free trial period will automatically renew to a paid subscription. * Subscription payments will be charged to your iTunes account at confirmation of your purchase and upon commencement of each renewal term. * Choose from different subscription options. Upgrade to the Premium version and unlock all premium wallpapers, get regular content updates and remove ads! *Notifications to keep you informed when new live wallpapers are added to the app. *Photos - to import backgrounds you like and set them to your Lock Screen Get high-quality animations for your screen and make your device draw admiring glances!įor complete access to all Live Wallpapers for Me features, you will need to allow access to the following: Have the wallpapers you liked at your fingertips to customize your lock screen anytime you want. You can also create a personal collection of wallpapers of your choice in a tap! Save and store them in the “Favorite” category. Press your Lock Screen to let the magic happen! Set the Live background you selected as your Lock Screen wallpaper Choose from impressive live wallpapers and press Save Here’s how to WOW everyone with the new look of your device: We add new great animations and dynamic themes weekly, on the fly, so you’ll always have something new for your screen at hand.Īlso, we have something special for you! With our Live Wallpaper Maker tool, you can turn your videos into live wallpapers and keep happy memories alive on your device's Lock Screen! ![]() You can even witness a powerful cosmic explosion right on your Lock Screen! Ready to take a step up in customizing your device? 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Fill it to your heart’s content then, review every job experience bullet point individually and ask, “Does this point show my expertise and say something new?” If the answer’s no, exclude it. How can your entire career fit into four or five bullet points for each job you’ve had? Here’s some good news: if you’re a senior engineer, it’s totally cool to drop from your resume the server job you had in college.Įven if you whittled the jobs you’d include down to three or four, try starting with a basic resume outline. The most painful part of building a resume is editing yourself. Instead, why not leverage a cover letter maker to explain some of those details you had to leave off your resume? You can also customize almost everything about your modern resume template, and make it your own. While you might get away with a two-page resume if you’ve got 10-plus years of experience under your hat, keep in mind that recruiters and hiring managers aren’t giving your resume the time of day it deserves anyway. To download and use it, click the Create button and a new file will be created for you, using that template, all ready for you to customize with your information. (C ough cough We won’t say his real name, but one of our team members whose title rhymes with re-bounder once sent out 20 resumes with his name spelled ‘Stepen.’) When you select a template youll be presented with a dialog box that shows you a larger view of the template. But you’d be aghast (fun word) at the number of folks rejected for a job because of this. Include those projects on your resume.Īvoid grammar and spelling errors. ![]() As you read, see if any prior work projects come to mind. Tailor your resume for each job you apply to, which means reading each job description carefully. ![]() Your work experience should focus on your measurable accomplishments, not on job responsibilities. Use numbers to showcase your impact in past roles. There are plenty of resume tips we could give you, but here are three that, when followed, will most increase your chances of snagging an interview: ![]() Would you believe we’ve actually seen resumes without a name?! Besides discovering disheartening news like that, we’ve figured out how to make a good resume. Not to blast our own horns, but we’ve reviewed tens of thousands of resumes in the last few years. ![]() The quantification plot provides valuable insights into the Western blot results. The control group will serve as a reference, and any changes in the treatment group can be easily observed. The graph will demonstrate the relative expression levels of the protein of interest in the control and treatment groups. With the calculated ratios and normalized values, we can plot a graph to Visualize the quantification results. By normalizing, we can compare the relative expression levels of the protein of interest between different samples or treatment groups. This normalization is essential to account for any variations in sample loading and ensure accurate quantification. To normalize the protein of interest, we divide its ratio by the ratio of the housekeeping proteins. Normalizing the Protein of Interest to Housekeeping Proteins Calculation of ratios should be performed for both the housekeeping genes and the protein of interest in both the control and treatment groups. By dividing the intensity values of the bands of interest by the control value, we can obtain ratios that represent the relative expression levels. In this case, the control value is derived from the housekeeping or actin bands. This involves dividing the intensity values of the bands by a control value. The next step in quantifying Western blots is calculating the ratios. Once the selection is made, we can proceed to the analysis phase. It is crucial to ensure that the selection includes all the bands of interest and that there is enough room underneath for further analysis. The selection should encompass the bands while avoiding unnecessary areas. This is done by using the selection tool, often represented by a rectangle icon. Once the image has been edited to perfection, we can proceed to select the bands of interest for analysis. It may require some trial and error to find the optimal settings. Using the "Adjust" option in the "Image" menu, we can navigate to "Brightness and Contrast" and make adjustments until the background is no longer visible. This can be done by adjusting the brightness and contrast of the image. In addition to aligning the bands, it is often necessary to remove the background from the image to obtain clear curves for analysis. Experimenting with different rotation angles will help achieve the desired alignment. If the bands are still slightly tilted, we can use the "Transform" option in the "Image" menu to rotate the image by a specific degree. First, we can invert the image by going to the "Edit" menu and selecting "Invert." This will ensure that the bands appear in a straight line. To correct any alignment issues with the bands, we can use the editing functions of the image software. Once the image is uploaded, it may appear with some imperfections, such as bands that are not aligned or a background that needs to be removed.Įditing the Image: Inverting and Rotating There are two ways to do this - either by going to "File" and selecting "Open" or simply dragging the image into the software. To begin the quantification process, the first step is to upload the image into the image software. Image software allows for accurate and efficient quantification of Western blots by measuring the intensity of the bands. Quantifying the intensity of the bands on a Western blot can provide valuable information about protein expression levels. Western blotting is a widely used technique in molecular biology to detect and analyze specific proteins in a biological sample. In this article, we will discuss how to perform the quantification of Western blots using image software. Normalizing the Protein of Interest to Housekeeping Proteins.Editing the Image: Inverting and Rotating.Easy Techniques for Western Blot Quantification Using ImageJ |
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