The interesting detail is in how quickly the dogs located the person. When it came to only one cue at a time, the sight, sound, and smell situations were each about equally effective in helping the dog, although none was as effective as all three cues at once. They timed the dog in each situation to see how long it took him to find the person in the testing room and get a treat.Īs you would expect, the dogs found the person almost every time when they had all three cues – sight, sound, and smell. They tested 11 dogs in an experiment that allowed the dogs to detect the presence of a person by smell only, sight only, sound only, or using all three senses at once. Megumi Fukuzawa and Marina Watanabe looked at how sight, sound, and smell can cue dogs to a person’s presence. Have you ever wondered which would be easier for him to understand, spoken words or visual cues? What is the most effective way of communicating with your dog? Recent research from Nihon University, in Japan, helps answer this question. But no doubt, you do a lot of talking to your dog, too. (5) They don't hear the doorbell (or the UPS truck).You may have seen deaf dogs responding to the hand signals of their owners, and perhaps, you use hand signals with your own dog. (4) They don't hear other dogs barking in the neighborhood, so they don't chime in. (3) They watch body language more acutely than hearing dogs. (2) They sleep soundly - most noises will not wake them, but the VIBRATIONS from others might. I do involve owners in the handling of their pets, both in studio and on location, but for reinforcement purposes, I like to understand how they "speak" to their dog.ĭeaf dogs bring certain advantages to the table: I will ask a client to show me the nuances of their own signals, when working with their dog to capture a great photograph. I have found that clients who have deaf dogs, often have a series of hand signals they use, much like ours, to make requests. Using their signs, and body language, they have no problem complying with STAY STILL requests for portraits. I rarely have a problem photographing our deaf dogs. He is showing her the reward she will receive when she completes the task. Stanton is asking Talley to STAY, and has already started to back up. Lacy shows Talley that sign, above, when she has met the command requested. In our house, thumbs up means "good" or "okay" to our deaf dogs. Pets and children also have to be taught how to respect each other. The video below is a good representation of most of the signs we use, and I like that a child is involved in the process, because I firmly believe kids need to be taught early how to help care for a pet. We parlay some of these very same signs into our deaf-dog vocabulary, as well as American Sign Language and things we just made up to suit our own dogs. Hand signals are often used in dog training, even for hearing dogs. Signs for quiet, sit, stay, down, wait, outside, eat, finished/no more, no, leave it/drop it, walkies.all of these and more are a part of our daily lives.īut signs aren't so far fetched. It has not only helped us to move forward with our two additional deafies (our Border Collie, Piper, and our recent addition, Talley, an English Setter), but has given us a lot of insight into the importance of routine, schedules, and body language. Burton is credited with being our first bilaterally deaf adoption, and he taught us so much of what we needed to know. Two are still with us, but we lost one (Burton, pictured near the end of this blog) a bit over a year ago at age 11.
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Our transformed minds are by grace, not our own wisdom, so we have no place to boast. It is true that our new life in Christ can convict them, Paul gets at this in Ephesians 5:3-21, but we have no business parading a self-righteous attitude in our rejection of homosexuality, divorce, abortion, etc. They hate us because we testify to the truth of Christ and they do not believe (1 Tim 1:13). The world is not to hate us because we are offensive in and of ourselves the offense is in the gospel (1 Cor. There is a big difference between these two. The world will hate Christians because we believe in Christ (John 3:19-20 2 Cor. The world's hatred of the church is not to be because the church represents morality. This is not the origin of ‘culture wars' because the people of God are neither to create their own ‘culture' nor are they to defend a conservative agenda for American (or European or English) culture. God tells us here that he intends the wicked to hate the righteous. The animosity we experience from unbelievers is sin, but it is divinely ordained hatred. What is important to note is that the enmity does not merely arise, it is specifically said to be set or appointed by God himself. This Hebrew word for ‘enmity' is used just five times in the OT (see Ez 25:15, hostility 35:5 Num 35:21-22, hatred unto murder). Moses places ‘enmity' first for the sake of emphasizing it. The normal structure of a Hebrew sentence begins with the verb but here the noun comes first (enmity). These elements of this first prophecy can be put under three categories: two seeds in humanity, judgment on Satan, and the victory of the Savior. There are seven things we will look at in this verse: (1) enmity between the seeds, (2) the source of the enmity, (3) the identity of the serpent, (4) the meaning of ‘seed,' (5) the identity of the woman's seed, (6) the doom of the serpent's seed, and (7) the suffering of the woman's seed. The gospel is spoken in Scripture for the first time from the Creator and Redeemer himself. In the midst of uttering a curse upon the serpent, God also prophetically announces the first promise of the gospel. God is the first prophetic voice of Scripture.īut v.15 is even more than that. Before God addresses Adam and Eve to curse them for their rebellion against himself in the covenant of works (3:16-19), God curses Satan in what is a foretelling of what will be the history of humanity. God is speaking to the serpent (3:14) about the new order for the universe after Adam's fall into sin (3:1-7). "Enmity I will put between you and the woman, PROTOEVANGELIUM OF GENESIS 3:15 By Dante Spencer
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Fill it to your heart’s content then, review every job experience bullet point individually and ask, “Does this point show my expertise and say something new?” If the answer’s no, exclude it. How can your entire career fit into four or five bullet points for each job you’ve had? Here’s some good news: if you’re a senior engineer, it’s totally cool to drop from your resume the server job you had in college.Įven if you whittled the jobs you’d include down to three or four, try starting with a basic resume outline. The most painful part of building a resume is editing yourself. Instead, why not leverage a cover letter maker to explain some of those details you had to leave off your resume? You can also customize almost everything about your modern resume template, and make it your own. While you might get away with a two-page resume if you’ve got 10-plus years of experience under your hat, keep in mind that recruiters and hiring managers aren’t giving your resume the time of day it deserves anyway. To download and use it, click the Create button and a new file will be created for you, using that template, all ready for you to customize with your information. (C ough cough We won’t say his real name, but one of our team members whose title rhymes with re-bounder once sent out 20 resumes with his name spelled ‘Stepen.’) When you select a template youll be presented with a dialog box that shows you a larger view of the template. But you’d be aghast (fun word) at the number of folks rejected for a job because of this. Include those projects on your resume.Īvoid grammar and spelling errors. As you read, see if any prior work projects come to mind. Tailor your resume for each job you apply to, which means reading each job description carefully. Your work experience should focus on your measurable accomplishments, not on job responsibilities. Use numbers to showcase your impact in past roles. There are plenty of resume tips we could give you, but here are three that, when followed, will most increase your chances of snagging an interview: Would you believe we’ve actually seen resumes without a name?! Besides discovering disheartening news like that, we’ve figured out how to make a good resume. Not to blast our own horns, but we’ve reviewed tens of thousands of resumes in the last few years. The quantification plot provides valuable insights into the Western blot results. The control group will serve as a reference, and any changes in the treatment group can be easily observed. The graph will demonstrate the relative expression levels of the protein of interest in the control and treatment groups. With the calculated ratios and normalized values, we can plot a graph to Visualize the quantification results. By normalizing, we can compare the relative expression levels of the protein of interest between different samples or treatment groups. This normalization is essential to account for any variations in sample loading and ensure accurate quantification. To normalize the protein of interest, we divide its ratio by the ratio of the housekeeping proteins. Normalizing the Protein of Interest to Housekeeping Proteins Calculation of ratios should be performed for both the housekeeping genes and the protein of interest in both the control and treatment groups. By dividing the intensity values of the bands of interest by the control value, we can obtain ratios that represent the relative expression levels. In this case, the control value is derived from the housekeeping or actin bands. This involves dividing the intensity values of the bands by a control value. The next step in quantifying Western blots is calculating the ratios. Once the selection is made, we can proceed to the analysis phase. It is crucial to ensure that the selection includes all the bands of interest and that there is enough room underneath for further analysis. The selection should encompass the bands while avoiding unnecessary areas. This is done by using the selection tool, often represented by a rectangle icon. Once the image has been edited to perfection, we can proceed to select the bands of interest for analysis. It may require some trial and error to find the optimal settings. Using the "Adjust" option in the "Image" menu, we can navigate to "Brightness and Contrast" and make adjustments until the background is no longer visible. This can be done by adjusting the brightness and contrast of the image. In addition to aligning the bands, it is often necessary to remove the background from the image to obtain clear curves for analysis. Experimenting with different rotation angles will help achieve the desired alignment. If the bands are still slightly tilted, we can use the "Transform" option in the "Image" menu to rotate the image by a specific degree. First, we can invert the image by going to the "Edit" menu and selecting "Invert." This will ensure that the bands appear in a straight line. To correct any alignment issues with the bands, we can use the editing functions of the image software. Once the image is uploaded, it may appear with some imperfections, such as bands that are not aligned or a background that needs to be removed.Įditing the Image: Inverting and Rotating There are two ways to do this - either by going to "File" and selecting "Open" or simply dragging the image into the software. To begin the quantification process, the first step is to upload the image into the image software. Image software allows for accurate and efficient quantification of Western blots by measuring the intensity of the bands. Quantifying the intensity of the bands on a Western blot can provide valuable information about protein expression levels. Western blotting is a widely used technique in molecular biology to detect and analyze specific proteins in a biological sample. In this article, we will discuss how to perform the quantification of Western blots using image software. Normalizing the Protein of Interest to Housekeeping Proteins.Editing the Image: Inverting and Rotating.Easy Techniques for Western Blot Quantification Using ImageJ However, if the complaint only says "I broke my arm and it was his fault," it could be dismissed on demurrer. If plaintiff pleads those things, he has pled sufficiently. So he could say that defendant owned a store and as the owner, he had a duty to keep the sidewalk free from ice, but he didn't bother to de-ice the sidewalk (thus breaching the duty), and plaintiff slipped on the ice and fell, breaking his arm and causing him to incur $1,500 in medical bills. The plaintiff's complaint must show facts indicating that there was a duty, that it was breached, that the breach caused his injuries and that he was damaged. To claim negligence, the plaintiff must show that the defendant (the person he's suing) had a duty, and he breached that duty, and his breach of that duty cased plaintiff's injuries and plaintiff suffered damages as a result. For instance, a plaintiff may sue someone for personal injury on a theory of negligence. The header identifies the court, the parties, and the case number. At a minimum, a motion to dismiss should have: 3 Header information. Try to find samples specific to your state, as the form and substance of motions can differ somewhat from state to state. The most common reason for filing a motion to dismiss is that the plaintiff has "failed to state a claim upon which relief can be granted," meaning that the filing fails to state a claim on which the judge can act - the demurrer, as described above. Type Motion to Dismiss and then your state. We have reviewed our content for bias and company-wide, we routinely meet with national experts to educate ourselves on better ways to deliver accessible content.įor 15 years our company has published content with clear steps to accomplish the how, with high quality sourcing to answer the why, and with original formats to make the internet a helpful place. Our commitment is to provide clear, original, and accurate information in accessible formats. We are incredibly worried about the state of general information available on the internet and strongly believe our mission is to give voice to unsung experts leading their respective fields. Our content experts ensure our topics are complete and clearly demonstrate a depth of knowledge beyond the rote. We perform original research, solicit expert feedback, and review new content to ensure it meets our quality pledge: helpful content – Trusted, Vetted, Expert-Reviewed and Edited. We write helpful content to answer your questions from our expert network. His work has even appeared on the front page of Reddit.Īrticles he's written have been used as a source for everything from books like Team Human by Douglas Rushkoff, media theory professor at the City University of New York's Queens College and CNN contributor, to university textbooks and even late-night TV shows like Comedy Central's with Chris Hardwick. His roundups of new features in Windows 10 updates have been called "the most detailed, useful Windows version previews of anyone on the web" and covered by prominent Windows journalists like Paul Thurrott and Mary Jo Foley on TWiT's Windows Weekly. Instructional tutorials he's written have been linked to by organizations like The New York Times, Wirecutter, Lifehacker, the BBC, CNET, Ars Technica, and John Gruber's Daring Fireball. The news he's broken has been covered by outlets like the BBC, The Verge, Slate, Gizmodo, Engadget, TechCrunch, Digital Trends, ZDNet, The Next Web, and Techmeme. Beyond the column, he wrote about everything from Windows to tech travel tips. He founded PCWorld's "World Beyond Windows" column, which covered the latest developments in open-source operating systems like Linux and Chrome OS. He also wrote the USA's most-saved article of 2021, according to Pocket.Ĭhris was a PCWorld columnist for two years. Beyond the web, his work has appeared in the print edition of The New York Times (September 9, 2019) and in PCWorld's print magazines, specifically in the August 2013 and July 2013 editions, where his story was on the cover. With over a decade of writing experience in the field of technology, Chris has written for a variety of publications including The New York Times, Reader's Digest, IDG's PCWorld, Digital Trends, and MakeUseOf. Chris has personally written over 2,000 articles that have been read more than one billion times-and that's just here at How-To Geek. Chris Hoffman is the former Editor-in-Chief of How-To Geek. Open in Google Sheets → Life Tracking Spreadsheet Templates Recipe and meal tracking templates Massive meal planning and nutrition tools spreadsheet Organize and easily update contact, share with colleagues. Open in Google Sheets → Contact list and CRM Google templates CRM Google Spreadsheet Track your project and customize your list of statuses and priorities. Streamline your project management process with multiple tasks and deliverables with this template from Smartsheet. View in Google Sheets → Google Sheet project management templates Project management spreadsheet template View in Google Sheets →Ī Gantt chart’s visual timeline allows you to see details about each task as well as project dependencies. 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Most of Claude Monet’s paintings were landscapes. National Gallery of Art, Washington D.C.In the US, the following art museums own one of the Rouen Cathedral paintings: In Europe you can view a painting from the Rouen Cathedral Series at the following art museums: Rouen Cathedral, Facade (Sunset) – Claude Monet Painting You can view a painting from the Monet Rouen Cathedral series at the following art museums in France: You can view Monet’s La Grenouillèr at The Metropolitan Museum of Art in New York. Monet’s painting provides a wonderful impressionism snapshot of 19th century outings. Interestingly, both Monet’s and Renoir’s rendition of La Grenouillère are very similar. They captured the resort and the middle class people enjoying themselves. The two artists set up their easels outdoors and painted en-plein air. Young Renoir and Monet went together on a painting trip to La Grenouillère. It was located on the River Seine and just a quick train trip from Paris. In the 19th century, La Grenouillère was a popular resort with a spa, boating facilities and a floating café. You can view Claude Monet’s Impression, Sunrise at the wonderful Musée Marmottan Monet in Paris. This antagonistic comment stuck and this new and ridiculed art movement finally had a name. An aggressive French art critic, Louis Leroy, reviewed this exhibition and accused the group of painting nothing but impressions. On April 1874 this painting and other artworks were displayed at the first major Impressionism Exhibition. One of the most famous Monet Paintings: Impression, Sunrise 1872 When asked for the title of this painting, Monet thought that an impression of Le Havre would be more fitting since it showed no specific elements. In this painting, Monet depicted the port city of Le Havre in Normandy, which was also his hometown. Although Monet painted over 2000 artworks, below is a list of ten of the most famous Claude Monet paintings Impression, Sunrise 1872Ĭlaude Monet‘s Impression, Sunrise is the painting that gave the name to the French impressionism art movement. Their revolutionary ideas and style paved the way to what we call today Modern Art. This was a huge break away from traditional art. As a result, Monet and the impressionists painted with brighter and bolder colors. This enabled them to capture the light and its effects on nature. The impressionists also left their painting studios and set up easels outdoors. For this, they painted with thick, short brushstrokes. Together with other like-minded artists, the impressionists decided to do away with capturing the fine details in a subject matter and instead paint a broader “impression”. His painting entitled Impression, Sunrise gave the name to the revolutionary impressionism art movement. Ten Most Famous Monet Paintings that the World Adores!Ĭlaude Monet was a famous French artist of the 19th century. Home » Artists » Impressionists » Ten Most Famous Monet Paintings You Should Know |
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